expression n. 1.表現(xiàn),表示,表達(dá)。 2.詞句;語(yǔ)句,措辭,說(shuō)法。 3.表情,臉色,態(tài)度;腔調(diào),聲調(diào)。 4.【數(shù)學(xué)】式,符號(hào)。 5.(油的)榨出,壓榨。 6.【生物學(xué)】表現(xiàn)度。 emotional expression 表情。 a happy expression 妙言。 an odd expression 怪話(huà)。 the expression of the eye 眼睛表情,眼色。 a smiling expression 笑臉。 beyond [past] expression 形容不出,無(wú)法表達(dá)。 find expression (in) (在…中)表現(xiàn)出來(lái)…,表現(xiàn)在…。 give expression to 表達(dá),反映(give the fullest expression to their initiative 充分表現(xiàn)他們的積極性)。 expression mark 【音樂(lè)】表示感情色彩的符號(hào)。 adj. -al 表現(xiàn)的;表情的。 n. -ism 【美術(shù)】表現(xiàn)主義;表現(xiàn)派。 -ist 1.n. 【美術(shù)】表現(xiàn)主義者,表現(xiàn)派。 2. 表現(xiàn)派的〔亦作 expressionistic〕。 adj. -less 缺乏表情的,呆板的。
In our study we have cloned the osd gene from s . typhimurium by pcr , characterized the gene product and used this gene to construct asd + expression cloning vectors ptrc99a - asd 再將hpylori尿素酶b亞單位基因與尿素酶b和熱休克蛋白a融合基因分別克隆入ptrc99a一asd質(zhì)粒的多克隆位點(diǎn)之內(nèi)。
Conclusion constructed the high - level expression clone of echistatin in e . coli . the expression of recombinant protein is higher , it make the further study of echistatin feasible 結(jié)論成功構(gòu)建了echistatin的原核高效表達(dá)克隆,表達(dá)量高于現(xiàn)有國(guó)內(nèi)外研究水平,為echistatin功能及相關(guān)疾病的研究奠定了基礎(chǔ)。
In the first part , the focus is to find the receptor molecules directly by screening two cdna libraries with a recombinant construct prpap or as an alternative , to find an enriched area in the embryo brains and construct libraries from this brain region and perform the expression cloning as above 方法: ( )以融合蛋白prpap通過(guò)瞬時(shí)表達(dá)克隆法篩選兩個(gè)cdna文庫(kù),或者通過(guò)與胚胎腦的結(jié)合實(shí)驗(yàn)篩選有豐富結(jié)合蛋白的腦區(qū),以圖構(gòu)建cdna文庫(kù)并進(jìn)行表達(dá)克隆的篩選。
Objective : construct high - level expression system of echistatin in e . coli methods : obtain amino - acid sequence of echistatin from genebank database . considering the bias of usage of 61 available aminoacid codons in e . coli , design the coding sequence of echistatin , synthesize the dna sequence chemically , get single copy coding gene and repeated two copy coding gene of echistatin . insert the sequence into expression vector pbv220 , and more , we construct fusion expression clone of echistatin with pcr , identify the recombinant vector by dna sequencing 目的構(gòu)建蛇毒鋸鱗蝰素( echistatin )的原核高效表達(dá)體系方法由genebank數(shù)據(jù)庫(kù)檢索蛇毒鋸鱗蝰素( echistatin )的氨基酸序列,結(jié)合大腸桿菌蛋白質(zhì)合成體系對(duì)氨基酸密碼子使用的偏愛(ài)性,設(shè)計(jì)了echistatin編碼基因,體外人工合成編碼基因dna片段,通過(guò)適當(dāng)?shù)南拗菩詢(xún)?nèi)切酶位點(diǎn)插入表達(dá)載體pbv220 ,分別構(gòu)建了echistatin的單拷貝表達(dá)克隆、雙拷貝串聯(lián)表達(dá)克?。贿M(jìn)一步通過(guò)pcr技術(shù)構(gòu)建echistatin的融合表達(dá)基因克隆。
百科解釋
Expression cloning is a technique in DNA cloning that uses expression vectors to generate a library of clones, with each clone expressing one protein. This expression library is then screened for the property of interest and clones of interest recovered for further analysis.